Journal: The Journal of Neuroscience

Article Title: Increased Neuronal Expression of the Early Endosomal Adaptor APPL1 Replicates Alzheimer’s Disease-Related Endosomal and Synaptic Dysfunction with Cholinergic Neurodegeneration

doi: 10.1523/JNEUROSCI.2331-24.2025

Figure Lengend Snippet: APPL1 transgene expression in the brain. A , Representative Western blot comparing APPL1 expression in various brain regions (CTX, cortex; HIP, hippocampus; CBL, cerebellum; RB, remaining brain) from 4- to 5-month-old nontransgenic littermate (non-Tg) and Thy1-APPL1 transgenic (Thy1-APPL1) mice. Overexpressed flag-tagged APPL1 proteins were detected in all brain regions in Thy1-APPL1 mice. B , Representative brain section immunolabeling with an anti-APPL1 antibody at low magnification (scale bar, 1 mm) demonstrates the APPL1 overexpression patterns in various brain regions of Thy1-APPL1 mice. C , Representative immunofluorescent images at low (40×) and high (120×) magnification show APPL1 (green) and NeuN (red) with DAPI (blue) staining in cortex layer V (scale bar, 10 µm; yellow arrows indicate neurons, white arrowheads identify non-neuronal cells; genotype as indicated). D , Western blots probed with anti-APPL1 and anti-rab5 antibodies of both non-Tg and Thy1-APPL1 brain homogenate prepared from mice of the indicated ages. E , Western blot analysis quantification showing increased APPL1 in the brain homogenates of Thy1-APPL1 mice compared with non-Tg mice at the indicated ages. ** p < 0.01, *** p < 0.001, two-tailed, unpaired t test. Data shown as mean ± SEM.

Article Snippet: For protein analyses, mouse hemibrains were homogenized in 1:10 (brain weight: buffer), pH 7.4, buffer containing 250 mM sucrose, 20 mM Tris-HCl, 1 mM EDTA, 1 mM EGTA, protease/phosphatase inhibitors ( ; ), and Western blot analyses as described previously and were performed with antibodies against APP (C1/6.1; 1 μg/ml; ), APP-βCTF (M3.2; 2 μg/ml; ), APPL1 (Proteintech; 1:1,000), rab5 (Abcam; 1:2,000), synaptophysin (Sigma-Aldrich, catalog #S5768, RRID:AB_477523; 1:2,000; ), phosphorylated tau [paired-helical filament tau, phosphorylated at Ser396 and Ser404 site (PHF1); 1:1,000, kind gift from P. Davies; ], and total tau protein (Agilent, catalog #A0024, RRID:AB_10013724; 1:5,000; ).

Techniques: Expressing, Western Blot, Transgenic Assay, Immunolabeling, Over Expression, Staining, Two Tailed Test

Journal: The Journal of Neuroscience

Article Title: Increased Neuronal Expression of the Early Endosomal Adaptor APPL1 Replicates Alzheimer’s Disease-Related Endosomal and Synaptic Dysfunction with Cholinergic Neurodegeneration

doi: 10.1523/JNEUROSCI.2331-24.2025

Figure Lengend Snippet: Early endosome alterations in Thy1-APPL1 mice. A , Representative immunofluorescent images of active, GTP-bound rab5 (rab5-GTP; red) and APPL1 (green) in neurons (layer V, prefrontal cortex) of non-Tg and Thy1-APPL1 mice at 12–13 months of age. Shown are images at lower magnification (40×, merged). The white arrow indicating the neuron shown at higher magnification for individual and merged immunofluorescent signal. Scale bar, 10 µm. B , Quantification of the average number, size, and total area of Rab5-GTP immunolabeled endosomes per cortical neuron in non-Tg versus Thy1-APPL1 mice at the indicated ages. Representative electron microscopy images containing dendritic profiles in layer V of prefrontal cortex ( C ) and hippocampus ( D ) regions and ( E ) quantification of average size and circumference of endosomes in both cortex and hippocampi showing the enlargement in Thy1-APPL1 compared with non-Tg mice aged at 12–13 months (red asterisk indicating endosome; 7 mice each genotype for cortex and 4 mice each genotype for hippocampi were used for quantification. Scale bar, 500 nm). Sixty images/mouse were quantified in E ; for cortex sections, a total of 2,635 endosomes from 14 mice were counted, averaging 188 endosomes per mouse; for hippocampal sections, a total of 1,594 endosomes were counted from 8 mice, with an average of 199 endosomes per mouse. F , Representative immunoelectron microscopy images containing dendritic profiles in layer V of prefrontal cortex labeled with rabbit anti-rab5 and mouse anti-APPL1 followed by gold-conjugated secondary anti-rabbit (6 nm, green arrow) and anti-mouse (10 nm, red arrow) in non-Tg and Thy1-APPL1 mice. Scale bar, 100 nm. G , Percentage of rab5/APPL1 double-positive endosomes versus total rab5-positive endosomes in the dendritic region of the cortex and hippocampus significantly increased in Thy1-APPL1 mice (3 mice per genotype. 35–40 images/mouse were used for counting; for cortex sections, a total of 130 endosomes were counted from 6 mice, with an average of 22 endosomes per mouse; for hippocampal sections, a total of 121 endosomes were counted from 6 mice, with an average of 20 endosomes per mouse). * p < 0.05, ** p < 0.01, *** p < 0.001, two-tailed, unpaired t test. Data is shown as mean ± SEM.

Article Snippet: For protein analyses, mouse hemibrains were homogenized in 1:10 (brain weight: buffer), pH 7.4, buffer containing 250 mM sucrose, 20 mM Tris-HCl, 1 mM EDTA, 1 mM EGTA, protease/phosphatase inhibitors ( ; ), and Western blot analyses as described previously and were performed with antibodies against APP (C1/6.1; 1 μg/ml; ), APP-βCTF (M3.2; 2 μg/ml; ), APPL1 (Proteintech; 1:1,000), rab5 (Abcam; 1:2,000), synaptophysin (Sigma-Aldrich, catalog #S5768, RRID:AB_477523; 1:2,000; ), phosphorylated tau [paired-helical filament tau, phosphorylated at Ser396 and Ser404 site (PHF1); 1:1,000, kind gift from P. Davies; ], and total tau protein (Agilent, catalog #A0024, RRID:AB_10013724; 1:5,000; ).

Techniques: Immunolabeling, Electron Microscopy, Immuno-Electron Microscopy, Labeling, Two Tailed Test

Journal: The Journal of Neuroscience

Article Title: Increased Neuronal Expression of the Early Endosomal Adaptor APPL1 Replicates Alzheimer’s Disease-Related Endosomal and Synaptic Dysfunction with Cholinergic Neurodegeneration

doi: 10.1523/JNEUROSCI.2331-24.2025

Figure Lengend Snippet: Age-related impairments of synaptic plasticity and hippocampal-dependent memory deficit in Thy1-APPL1 mice. A–C , Non-Tg and Thy1-APPL1 mice at 7–9 months of age ( n = 5 for both genotype), input/output relationship plots ( A ), LTP by theta-burst stimulation (TBS) in the Schaffer collateral synapses (CA3-CA1) of hippocampal slices ( B ), and averages of fEPSP slopes at 1, 40, and 80 min following tetanic stimulation ( C ) of the hippocampal slices showing no differences between non-Tg and Thy1-APPL1 mice. D–F , Non-Tg and Thy1-APPL1 mice at 12–13 months of age ( n = 5 for both genotype), input/output relationship plots ( D ), LTP induced by TBS in the Schaffer collateral synapses (CA3-CA1) in hippocampal slices ( E ), and averages of fEPSP slopes at 1, 40, and 80 min ( F ) showing significant reduction in Thy1-APPL1 mice ( F (1,236) = 6.696, p = 0.0103 for the slope, linear regression). G–I , Non-Tg and Thy1-APPL1 mice at 12–13 months of age ( n = 5 for both genotypes), input/output relationship plots ( G ), LTD induced by low-frequency stimulation (LFS; H ) in hippocampal slices of Thy1-APPL1 ( F (1,127) = 14.83, p = 0.0002 for the intercepts, linear regression), and averages of fEPSP slopes at 50 min following LFS induction ( I ) in the hippocampal slices did not show expected reduction in Thy1-APPL1 mice. J , Recognition index at 3 h after familiarization indicated the memory deficit in Thy1-APPL1 mice at 12–13 months of age ( t (18) = 2.187, p = 0.042). * p < 0.05, *** p < 0.001, two-tailed, unpaired t test. Data is shown as mean ± SEM.

Article Snippet: For protein analyses, mouse hemibrains were homogenized in 1:10 (brain weight: buffer), pH 7.4, buffer containing 250 mM sucrose, 20 mM Tris-HCl, 1 mM EDTA, 1 mM EGTA, protease/phosphatase inhibitors ( ; ), and Western blot analyses as described previously and were performed with antibodies against APP (C1/6.1; 1 μg/ml; ), APP-βCTF (M3.2; 2 μg/ml; ), APPL1 (Proteintech; 1:1,000), rab5 (Abcam; 1:2,000), synaptophysin (Sigma-Aldrich, catalog #S5768, RRID:AB_477523; 1:2,000; ), phosphorylated tau [paired-helical filament tau, phosphorylated at Ser396 and Ser404 site (PHF1); 1:1,000, kind gift from P. Davies; ], and total tau protein (Agilent, catalog #A0024, RRID:AB_10013724; 1:5,000; ).

Techniques: Two Tailed Test

Journal: The Journal of Neuroscience

Article Title: Increased Neuronal Expression of the Early Endosomal Adaptor APPL1 Replicates Alzheimer’s Disease-Related Endosomal and Synaptic Dysfunction with Cholinergic Neurodegeneration

doi: 10.1523/JNEUROSCI.2331-24.2025

Figure Lengend Snippet: NGF retrograde signaling and involvement of APPL1 in healthy neurons and after a rise in neuronal APPL1 levels related to Alzheimer's disease in APPL1 overexpressing neurons. Schematic diagram depicts ( A ) the endocytosis of APP, NGF, and its receptor TrkA into a rab5 early endosome. TrkB mediation of BDNF signaling by APPL1 (data not shown) is considered to follow a similar sequence. B , Normal NGF signaling is facilitated by recruitment of APPL1, a direct TrkA ligand and adaptor for other signaling molecules mediating retrograde transport of a maturing endosome carrying the NGF signal to the nucleus to activate a neurotrophic transcriptional program supporting functioning of ChAT neurons and other NGF targets. In AD (not shown; see main text), abnormally elevated APP-βCTF levels arising via multiple possible mechanisms raise levels of the activated form of rab5 (rab5-GTP) on endosomal membranes, in part by recruiting more APPL1 to the endosome via the phosphotyrosine binding (PTB) domain of APPL1 . APPL1's greater affinity for rab5-GTP prolongs association of this activated form on the endosome, thus promoting a pathogenic rab5 hyperactivation leading to increased endocytosis, early endosomal fusion, and endosome enlargement, as depicted in C . C , Moderately elevating APPL1 selectively in neurons of Thy1-APPL1 mice phenocopies mouse models of APP-βCTF elevation or rab5 overexpression with respect to rab5 hyperactivation, abnormal endosome enlargement, stasis of endosome transport, synaptic plasticity deficits, and basal forebrain cholinergic neurodegeneration. These pathological effects, as observed in AD brain, also reflect impaired NGF/TrkA signaling and decreased expression of genes for neuronal survival, growth and differentiation ( ; ). Further information is provided in the text and in more detail in reviews ( ; ).

Article Snippet: For protein analyses, mouse hemibrains were homogenized in 1:10 (brain weight: buffer), pH 7.4, buffer containing 250 mM sucrose, 20 mM Tris-HCl, 1 mM EDTA, 1 mM EGTA, protease/phosphatase inhibitors ( ; ), and Western blot analyses as described previously and were performed with antibodies against APP (C1/6.1; 1 μg/ml; ), APP-βCTF (M3.2; 2 μg/ml; ), APPL1 (Proteintech; 1:1,000), rab5 (Abcam; 1:2,000), synaptophysin (Sigma-Aldrich, catalog #S5768, RRID:AB_477523; 1:2,000; ), phosphorylated tau [paired-helical filament tau, phosphorylated at Ser396 and Ser404 site (PHF1); 1:1,000, kind gift from P. Davies; ], and total tau protein (Agilent, catalog #A0024, RRID:AB_10013724; 1:5,000; ).

Techniques: Sequencing, Binding Assay, Over Expression, Expressing

Journal: The Journal of Neuroscience

Article Title: Increased Neuronal Expression of the Early Endosomal Adaptor APPL1 Replicates Alzheimer’s Disease-Related Endosomal and Synaptic Dysfunction with Cholinergic Neurodegeneration

doi: 10.1523/JNEUROSCI.2331-24.2025

Figure Lengend Snippet: Western blot analysis and synaptic vesicle endocytosis (SVE) revealed the abnormalities in hippocampal synaptosomes of Thy1-APPL1 mice. A , Representative Western blots from one of the two experiments showing various protein markers in hippocampal homogenates and hippocampal synaptosome preparations from non-Tg and Thy1-APPL1 mice ( n = 6 each genotype). Quantitation of these Western blots ( B , C ) shows significantly higher levels of APPL1, βCTF, PHF1, and the ratio of PHF1/total tau in synaptosomes of Thy1-APPL1 compared with non-Tg mice at 12–13 months of age. D , Representative fluorescent images of internalized FM4-64 (red) with corresponding CMFDA labeling of total synaptosomes (green) using hippocampal synaptosomes prepared from non-Tg and Thy1-APPL1 mice at 12–13 months of age. E , The ratio of internalized FM4-64 to CMDFA was significantly increased in hippocampal synaptosomes of Thy1-APPL1 mice at 12–13 months, but not in 7 months of age, indicating elevated endocytosis in older Thy1-APPL1 mice compared with non-Tg. F , Representative images of CMFDA (green) labeling followed by anti-rab5 (red) immunolabeling and ( G ) quantification of the intensity of rab5 to CMFDA showing an increase in rab5 immunosignal per CMFDA-labeled synaptosome in hippocampal synaptosomes of Thy1-APPL1 mice at 12–13 months of age. Scale bar, 5 µm, * p < 0.05, *** p < 0.001, two-tailed, unpaired t test. Data shown as mean ± SEM. Western blots of hippocampal synaptosome (10 µg of synaptosome protein, input; H ), and GTP-agarose pull-down of hippocampal synaptosome (200 µg of synaptosome protein) from non-Tg and Thy1-APPL1 mice ( I ) probed with anti-APPL1 and anti-rab5 antibodies, total protein stained by Revert 700 Total Protein Stain are also shown at the bottom of H and I . The APPL1 and rab5 band density in both input and pull-down against total protein in relation with non-Tg are presented in J , * p < 0.05, *** p < 0.001, one-way ANOVA. Data is shown as mean ± SEM.

Article Snippet: For protein analyses, mouse hemibrains were homogenized in 1:10 (brain weight: buffer), pH 7.4, buffer containing 250 mM sucrose, 20 mM Tris-HCl, 1 mM EDTA, 1 mM EGTA, protease/phosphatase inhibitors ( ; ), and Western blot analyses as described previously and were performed with antibodies against APP (C1/6.1; 1 μg/ml; ), APP-βCTF (M3.2; 2 μg/ml; ), APPL1 (Proteintech; 1:1,000), rab5 (Abcam; 1:2,000), synaptophysin (Sigma-Aldrich, catalog #S5768, RRID:AB_477523; 1:2,000; ), phosphorylated tau [paired-helical filament tau, phosphorylated at Ser396 and Ser404 site (PHF1); 1:1,000, kind gift from P. Davies; ], and total tau protein (Agilent, catalog #A0024, RRID:AB_10013724; 1:5,000; ).

Techniques: Western Blot, Quantitation Assay, Labeling, Immunolabeling, Two Tailed Test, Staining